Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia pastoris

Autor: Junhao Cheng, Mingkun Wu, Ren Zhong, Dayong Si, Geng Meng, Rijun Zhang, Yueping Zhang
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: BioTechniques, Vol 71, Iss 2, Pp 445-450 (2021)
Druh dokumentu: article
ISSN: 1940-9818
0736-6205
DOI: 10.2144/btn-2021-0039
Popis: The development of a quick, single-step cloning system for generation of multiexon gene expression constructs is presented. The system allows efficient and cost-effective assembly of multiple exons of interest genes into different expression plasmids in both Escherichia coli and Pichia pastoris. The high cloning efficiency and low cost of the system make it ideal for a novel workflow for the assembly of intron-bearing genes for expression in two different expression hosts.
Databáze: Directory of Open Access Journals