Antitumoral effects of Amblyomma sculptum Berlese saliva in neuroblastoma cell lines involve cytoskeletal deconstruction and cell cycle arrest

Autor: Thatyanne Gradowski do Nascimento, Priscilla Santos Vieira, Sheron Campos Cogo, Marcela Ferreira Dias-Netipanyj, Nilton de França Junior, Diana Aparecida Dias Câmara, Allan Saj Porcacchia, Ronaldo Zucatelli Mendonça, Andréa Novais Moreno-Amaral, Paulo Luiz de Sá Junior, Simone Michaela Simons, Luciana Zischler, Selene Elifio-Esposito
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2019
Předmět:
Zdroj: Revista Brasileira de Parasitologia Veterinária, Vol 28, Iss 1, Pp 126-133 (2019)
Druh dokumentu: article
ISSN: 1984-2961
41760301
DOI: 10.1590/s1984-296120180098
Popis: Abstract The antitumor properties of ticks salivary gland extracts or recombinant proteins have been reported recently, but little is known about the antitumor properties of the secreted components of saliva. The goal of this study was to investigate the in vitro effect of the saliva of the hard tick Amblyomma sculptum on neuroblastoma cell lines. SK-N-SK, SH-SY5Y, Be(2)-M17, IMR-32, and CHLA-20 cells were susceptible to saliva, with 80% reduction in their viability compared to untreated controls, as demonstrated by the methylene blue assay. Further investigation using CHLA-20 revealed apoptosis, with approximately 30% of annexin-V positive cells, and G0/G1-phase accumulation (>60%) after treatment with saliva. Mitochondrial membrane potential (Δψm) was slightly, but significantly (p < 0.05), reduced and the actin cytoskeleton was disarranged, as indicated by fluorescent microscopy. The viability of human fibroblast (HFF-1 cells) used as a non-tumoral control decreased by approximately 40%. However, no alterations in cell cycle progression, morphology, and Δψm were observed in these cells. The present work provides new perspectives for the characterization of the molecules present in saliva and their antitumor properties.
Databáze: Directory of Open Access Journals
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