| Autor: |
Leiming Wu, Mingliang Zhang, Ran Zhang, Haizhong Yu, Hailang Wang, Jingyang Li, Youmei Wang, Zhen Hu, Yanting Wang, Zi Luo, Lin Li, Lingqiang Wang, Liangcai Peng, Tao Xia |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Biotechnology for Biofuels, Vol 14, Iss 1, Pp 1-15 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1754-6834 |
| DOI: |
10.1186/s13068-021-02093-8 |
| Popis: |
Abstract Background As a major component of plant cell walls, cellulose provides the most abundant biomass resource convertible for biofuels. Since cellulose crystallinity and polymerization have been characterized as two major features accounting for lignocellulose recalcitrance against biomass enzymatic saccharification, genetic engineering of cellulose biosynthesis is increasingly considered as a promising solution in bioenergy crops. Although several transcription factors have been identified to regulate cellulose biosynthesis and plant cell wall formation, much remains unknown about its potential roles for genetic improvement of lignocellulose recalcitrance. Results In this study, we identified a novel rice mutant (Osfc9/myb103) encoded a R2R3-MYB transcription factor, and meanwhile generated OsMYB103L-RNAi-silenced transgenic lines. We determined significantly reduced cellulose levels with other major wall polymers (hemicellulose, lignin) slightly altered in mature rice straws of the myb103 mutant and RNAi line, compared to their wild type (NPB). Notably, the rice mutant and RNAi line were of significantly reduced cellulose features (crystalline index/CrI, degree of polymerization/DP) and distinct cellulose nanofibers assembly. These alterations consequently improved lignocellulose recalcitrance for significantly enhanced biomass enzymatic saccharification by 10–28% at p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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