Autor: |
Kürşat Er, Zübeyde Akin Polat, Fatih Özan, Tamer Taşdemir, Ufuk Sezer, Şeyda Hergüner Siso |
Jazyk: |
angličtina |
Rok vydání: |
2008 |
Předmět: |
|
Zdroj: |
Journal of the Formosan Medical Association, Vol 107, Iss 8, Pp 609-615 (2008) |
Druh dokumentu: |
article |
ISSN: |
0929-6646 |
DOI: |
10.1016/S0929-6646(08)60178-3 |
Popis: |
Background/Purpose: The aim of this study was to analyze the cytotoxicity of strontium ranelate (SR) on human periodontal ligament fibroblasts (PDL cells) in vitro. Methods: PDL cells were obtained from healthy human third molars and cultured in Dulbecco's Modified Eagle's Medium. The experimental groups were: G1, cultures treated with fresh medium (control); and G2, G3, G4 and G5: treated with SR at 20, 10, 5 and 2.5 mg/mL, respectively. The experimental times were 1, 6, 12 and 24 hours (short-term) for viability, and 2, 4, 6 and 8 days (long-term) for cell survival. The cells were counted using a hemocytometer. Data were then analyzed by one-way ANOVA and Tukey's tests (p < 0.05). Results: Cultures treated with the highest SR concentrations (G2 and G3) had significantly lower cell viability and cell numbers (p < 0.05) than those in G1, G4 and G5. SR at 2.5 mg/mL was non-cytotoxic to PDL cells. Conclusion: SR was non-toxic at appropriate concentrations. Preclinical tests are needed to further assess its safety and effectiveness for tooth resorption prior to clinical use. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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