| Autor: |
Alphonse Calenda, Nadine Mestre-Francés, Christian Czech, Laurent Pradier, Arlette Petter, Martine Perret, Noëlle Bons, Michel Bellis |
| Jazyk: |
angličtina |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
Neurobiology of Disease, Vol 5, Iss 5, Pp 323-333 (1998) |
| Druh dokumentu: |
article |
| ISSN: |
1095-953X |
| DOI: |
10.1006/nbdi.1998.0205 |
| Popis: |
A 1340-bp cDNA fragment encoding the lemurian presenilin 2 protein (PS2) was isolated from aMicrocebus murinusbrain cDNA library by PCR using oligonucleotide primers based on the nucleotide sequence of the human gene. Analysis of five isolated clones showed that the sequence encoded a 448-amino-acid open reading frame, 95.5% identical to the human and 93.5% identical to the mouse presenilin 2 sequences. However, neither the localization of the 2 positions in PS2 nor that of the 43 positions in PS1 associated with early onset Alzheimer's disease were changed. Expression of the presenilin 2 was detected by RT–PCR and compared with that of presenilin 1 and βAPP in the brain and in peripheral tissues (liver, kidney, and spleen). Immunohistochemistry with a specific polyclonal antiserum raised against a synthetic peptide from the N-terminal part of the human PS2 showed that the protein is distributed throughout the microcebe brain, in vascular and nerve structures. In cortical and in subcortical areas, PS2 labeling was weak and granular in appearance and was scattered throughout the cytoplasm of many neurones, extending into neurites. The gene expression of PS2 increased with age but was not affected by the presence of numerous amyloid plaques. Double labeling immunocytochemistry detected very few neurones with combined immunoreactivity PS2 and APP, or PS2 and Tau. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
Full Text from ScienceDirect