| Autor: |
Nahuai Badiola, Cristina Malagelada, Nuria Llecha, Juan Hidalgo, Joan X. Comella, Josefa Sabriá, José Rodríguez-Alvarez |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
Neurobiology of Disease, Vol 35, Iss 3, Pp 438-447 (2009) |
| Druh dokumentu: |
article |
| ISSN: |
1095-953X |
| DOI: |
10.1016/j.nbd.2009.06.005 |
| Popis: |
TNF-α has been reported to be relevant in stroke-induced neuronal death. However the precise function of TNF-α in brain ischemia remains controversial since there are data supporting either a detrimental or a protective effect. Here we show that TNF-α is released after oxygen–glucose deprivation (OGD) of cortical cultures and is a major contributor to the apoptotic death observed without affecting the OGD-mediated necrotic cell death. In this paradigm, apoptosis depends on TNF-α-induced activation of caspase-8 and -3 without affecting the activation of caspase-9. By using knock-out mice for TNF-α receptor 1, we show that the activation of both caspase-3 and -8 by TNF-α is mediated by TNF-α receptor 1. The pro-apoptotic role of TNF-α in OGD is restricted to neurons and microglia, since astrocytes do not express either TNF-α or TNF-α receptor 1. Altogether, these results show that apoptosis of cortical neurons after OGD is mediated by TNF-α/TNF-α receptor 1. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect