Evaluation of SARS-CoV-2 viral RNA in fecal samples

Autor: Alvaro Mesoraca, Katia Margiotti, Antonella Viola, Antonella Cima, Davide Sparacino, Claudio Giorlandino
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Virology Journal, Vol 17, Iss 1, Pp 1-3 (2020)
Druh dokumentu: article
ISSN: 1743-422X
DOI: 10.1186/s12985-020-01359-1
Popis: Abstract The need for timely establishment of a complete diagnostic protocol of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is demanded worldwide. We selected 15 positive novel coronavirus disease 19 (COVID-19) patients with mild or no symptom. Initially, fecal samples were negative in the 67% (10/15) of the cases, while 33% (5/10) of the cases were positive. After serial virus RNA testing, 73% (11/15) of the cases resulted positive to fecal specimens. In particular, 15 days after the first positive respiratory specimens test, 6 fecal specimens became positive for SARS-CoV-2 RNA, while 13 respiratory test returned negative result. In conclusion, qRT-PCR assays of fecal specimens, is an important step to control infection, suggesting that samples remained positive for SARS-CoV-2 RNA longer time then respiratory tract samples. Our results enhance the recent knowledge on this emerging infectious disease and offer suggestions for a more complete diagnostic strategy.
Databáze: Directory of Open Access Journals
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