Association between HOTAIR rs1899663 G>T Gene Polymorphism and Thyroid Cancer Susceptibility
| Autor: | R Madadi Rad, N Pouladi, A Nemati Bosharani, M Alizadeh |
|---|---|
| Jazyk: | English<br />Persian |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 24, Iss 1, Pp 95-102 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1561-4107 2251-7170 |
| Popis: | Background and Objective: Studies show that single nucleotide polymorphisms of the HOTAIR gene are associated with a variety of cancers, including colorectal, breast, and thyroid cancers. The product of the HOTAIR gene is a long non-coding RNA that is involved in regulating gene expression, cell cycle and apoptosis and is considered an oncogene. The present study was conducted to investigate the association between HOTAIR rs1899663 G>T gene polymorphism and thyroid cancer susceptibility in northwestern Iran. Methods: In this case-control study, peripheral blood was obtained from 90 patients with thyroid cancer referred to Tabriz hospitals as well as 198 healthy individuals. After DNA extraction by saturated salt and proteinase K method, single nucleotide polymorphism of HOTAIR rs1899663 gene was examined by tetra-primers ARMS PCR. Then, allelic and genotypic frequencies of control and case groups were calculated and compared. Findings: In this study, the genotypic frequencies of GG, GT and TT in the case group were 23.3%, 49% and 27.7%, and in the control group were 20.2%, 41% and 38.8%, respectively. Allele frequencies of G and T were 48% and 52.2% in the case group and 41% and 59% in the control group, respectively. Allelic and genotypic comparisons between case and control groups showed no significant relationship. Conclusion: The results of the study showed that HOTAIR rs1899663 gene polymorphism is not associated with any of the clinicopathological features of thyroid cancer. |
| Databáze: | Directory of Open Access Journals |
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