Cytotoxic Effect of 4-Hydroxyisoleucine on Liver Cancer Cell Line
| Autor: | Masoumeh Babaei, Mohammad Reza Haeri, Seyede Zahra Mousavi, Mohammad Khazeni |
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| Jazyk: | perština |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 9, Iss 1, Pp 1-6 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1735-7799 2008-1375 |
| Popis: | Background and Objectives: 4-hydroxyisoleucine is an amino acid in Fenugreek, which has antidiabetic properties. This effect is possibly brought about by activation of PI3K. In this study, cytotoxic effect of 4-hydroxyisoleucine on hepatoma cell lines was studied. Methods: In this experimental study, HepG2 and Huh-7 cell lines were cultured in a 96-well microplate and after 24 hours incubation, cells were exposed to different concentrations of 4-hydroxyisoleucine (0.5-64mM). Cell viability was determined using MTT assay after 24, 48, and 72 hours of incubation. The results were analyzed using one-way ANOVA and Tukey’s test. Results: The percentage of cytotoxicity caused by 4-hydroxyisoleucine is concentration- and time-dependent. In this study, this difference was statistically significant compared to the control group (p |
| Databáze: | Directory of Open Access Journals |
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