A Strategy for the Identification of Differentially Expressed Proteins Secreted by Fibroblasts
| Autor: | Francesca Viti, Maja A. Bumke, Michela Silacci, Luciano Zardi, Dario Neri |
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| Jazyk: | German<br />English<br />French |
| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | CHIMIA, Vol 54, Iss 11 (2000) |
| Druh dokumentu: | article |
| ISSN: | 0009-4293 2673-2424 |
| Popis: | Monoclonal antibodies, which recognise markers of the modified tumour extracellular matrix, may be valuable tools for the diagnosis and therapy of cancer. The pattern of expression of some good-quality tumour-associated antigens is known to be regulated by intracellular pH. In this article, a strategy is described for the isolation of human antibodies specific for proteins which are differentially secreted by fibroblasts at different pH values. We panned a large synthetic antibody phage library against biotinylated proteins secreted by normal human dermal fibroblasts, cultured at pH 7.5, in the presence or in the absence of a molar excess of unbiotinylated proteins, secreted by fibroblasts cultured at pH 6.7. A panel of monoclonal antibodies was isolated, whose reactivity was tested by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry. |
| Databáze: | Directory of Open Access Journals |
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