Secretome signature of cardiopoietic cells echoed in rescued infarcted heart proteome

Autor: D. Kent Arrell, Ruben J. Crespo‐Diaz, Satsuki Yamada, Ryounghoon Jeon, Armin Garmany, Sungjo Park, Jeffrey P. Adolf, Christopher Livia, Matthew L. Hillestad, Jozef Bartunek, Atta Behfar, Andre Terzic
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Stem Cells Translational Medicine, Vol 10, Iss 9, Pp 1320-1328 (2021)
Druh dokumentu: article
ISSN: 2157-6580
2157-6564
DOI: 10.1002/sctm.20-0509
Popis: Abstract Stem cell paracrine activity is implicated in cardiac repair. Linkage between secretome functionality and therapeutic outcome was here interrogated by systems analytics of biobanked human cardiopoietic cells, a regenerative biologic in advanced clinical trials. Protein chip array identified 155 proteins differentially secreted by cardiopoietic cells with clinical benefit, expanded into a 520 node network, collectively revealing inherent vasculogenic properties along with cardiac and smooth muscle differentiation and development. Next generation RNA sequencing, refined by pathway analysis, pinpointed miR‐146 dependent regulation upstream of the decoded secretome. Intracellular and extracellular integration unmasked commonality across cardio‐vasculogenic processes. Mirroring the secretome pattern, infarcted hearts benefiting from cardiopoietic cell therapy restored the disease proteome engaging cardiovascular system functions. The cardiopoietic cell secretome thus confers a therapeutic molecular imprint on recipient hearts, with response informed by predictive systems profiling.
Databáze: Directory of Open Access Journals
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