Purinergic Receptors in Spinal Cord-Derived Ependymal Stem/Progenitor Cells and Their Potential Role in Cell-Based Therapy for Spinal Cord Injury
| Autor: | Rosa Gómez-Villafuertes Ph.D., Francisco Javier Rodríguez-Jiménez, Ana Alastrue-Agudo, Miodrag Stojkovic, María Teresa Miras-Portugal, Victoria Moreno-Manzano |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Cell Transplantation, Vol 24 (2015) |
| Druh dokumentu: | article |
| ISSN: | 0963-6897 1555-3892 59433086 |
| DOI: | 10.3727/096368914X682828 |
| Popis: | Spinal cord injury (SCI) is a major cause of paralysis with no current therapies. Following SCI, large amounts of ATP and other nucleotides are released by the traumatized tissue leading to the activation of purinergic receptors that, in coordination with growth factors, induce lesion remodeling and repair. We found that adult mammalian ependymal spinal cord-derived stem/progenitor cells (epSPCs) are capable of responding to ATP and other nucleotidic compounds, mainly through the activation of the ionotropic P2X 4 , P2X 7 , and the metabotropic P2Y 1 and P2Y 4 purinergic receptors. A comparative study between epSPCs from healthy rats versus epSPCis, obtained after SCI, shows a downregulation of P2Y 1 receptor together with an upregulation of P2Y 4 receptor in epSPCis. Moreover, spinal cord after severe traumatic contusion shows early and persistent increases in the expression of P2X 4 and P2X 7 receptors around the injury, which are completely reversed when epSPCis were ectopically transplanted. Since epSPCi transplantation significantly rescues neurological function after SCI in parallel to inhibition of the induced P2 ionotropic receptors, a potential avenue is open for therapeutic alternatives in SCI treatments based on purinergic receptors and the endogenous reparative modulation. |
| Databáze: | Directory of Open Access Journals |
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