| Autor: |
Adam L. Hopper, Claire L. Hudson, Diksha Klair, Qiao Ding, Zhujun Gao, Aprajeeta Jha, Austin Bryan, Rohan V. Tikekar, Timothy Coolong, Laurel L. Dunn, Shirley A. Micallef |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Frontiers in Plant Science, Vol 15 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1664-462X |
| DOI: |
10.3389/fpls.2024.1370495 |
| Popis: |
IntroductionWildlife feces can contaminate vegetables when enteric bacteria are released by rain and splashed onto crops. Regulations require growers to identify and not harvest produce that is likely contaminated, but U.S. federal standards do not define dimensions for no-harvest zones. Moreover, mulching, used to retain soil moisture and maximize crop yield may impact rain-mediated bacterial dispersal from feces.MethodsTo assess Escherichia coli dissemination from a fecal point source to lettuce grown on various mulches, lettuce cv. ‘Magenta’ was transplanted into raised beds with plastic, biodegradable plastic, straw, or left uncovered at field sites in Maryland and Georgia. Eleven days post-transplant, 10 g of rabbit manure spiked with ~8 log CFU g-1E. coli were deposited in each bed. One day following natural or simulated rain events, lettuce was sampled along 1.5 m transects on either side of fecal deposits. Lettuce-associated E. coli was semi-quantified with an MPN assay and dependence on fecal age (stale or fresh), lettuce age (baby leaf or mature head), distance from point source, mulch and post-rain days were statistically evaluated.ResultsDistance (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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