| Autor: |
Mohammad Shamim Hossein, Young-Bum Son, Yeon Woo Jeong, Yeon Ik Jeong, Mi Na Kang, Eun Ji Choi, Kang Bae Park, Yu Ra Bae, Dae Young Kim, Woo Suk Hwang |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Frontiers in Veterinary Science, Vol 10 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2297-1769 |
| DOI: |
10.3389/fvets.2023.1227202 |
| Popis: |
Propagation of transgenic animals by germline transmission using assisted reproductive technologies such as in vitro fertilization (IVF) is the most efficient way to produce transgenic colonies for biomedical research. The objective of this study was to generate transgenic puppies from a founder dog expressing the mutated human amyloid precursor protein (mhAPP) gene. Experiment I assessed the characteristics of the semen prepared by freshly diluted, swim-up, and Percoll gradient methods using a computer-assisted semen analyzer (CASA). Motile and progressively motile sperm counts were higher in the Percoll gradient samples (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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