| Autor: |
Tiphaine Birien, Axel Thiel, Ghislaine Henneke, Didier Flament, Yann Moalic, Mohamed Jebbar |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Genes, Vol 9, Iss 2, p 77 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2073-4425 |
| DOI: |
10.3390/genes9020077 |
| Popis: |
A gene disruption system for Thermococcus barophilus was developed using simvastatin (HMG-CoA reductase encoding gene) for positive selection and 5-Fluoroorotic acid (5-FOA), a pyrF gene for negative selection. Multiple gene mutants were constructed with this system, which offers the possibility of complementation in trans, but produces many false positives ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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