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Dipsacus asper wall (DA) is an ancient Chinese medicinal material that has long been used to maintain the health of human bones. The present study aimed to evaluate the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) of Dipsacus asper wall extracts (DAE). Microwave-assisted alcohol extraction of 100 mesh DA powder under optimal conditions can obtain 58.66% (w/w) yield of the crude extract. PDLSCs have excellent differentiation potential. PDLSCs treated with DA extract (DAE) underwent osteogenesis, exhibiting a higher expression of the Col-1, ALP, Runx2, and OCN genes, and had a 1.4-fold increase in mineralization, demonstrating the potential of DAE to promote osteogenic differentiation. After the addition of PI3K inhibitor LY294002, the expression of osteogenic genes was significantly inhibited, confirming that PI3K is an important pathway for DAE to induce osteogenesis. Mix DAE with polycaprolactone/polyethylene glycol (PCL/PEO) to obtain nanofibers with a diameter of 488 nm under optimal electrospinning conditions. The physical property analysis of nanofibers with and without DAE includes FTIR, mechanical strength, biodegradability, swelling ratio and porosity, and cell compatibility. When cells induced by nanofibers with or without DAE, the mineralization of PDLSCs cultured on PCL/PEO/DAE was 2.6-fold higher than that of PCL/PEO. The results of the study confirm that both DAE and PCL/PEO nanofibers have the effect of promoting osteogenic differentiation. In order to obtain the best induction effect, the optimal amount of DAE can be discussed in future research. |
