Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis
| Autor: | Patrícia Longuinhos Peixoto, Evaldo Nascimento, Guilherme Grossi Lopes Cançado, Rodrigo Rodrigues Cambraia de Miranda, Regina Lunardi Rocha, Ricardo Nascimento Araújo, Ricardo Toshio Fujiwara |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Memorias do Instituto Oswaldo Cruz, Vol 106, Iss 2, Pp 200-206 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1678-8060 0074-0276 |
| DOI: | 10.1590/S0074-02762011000200014 |
| Popis: | In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we found that the crude extract of the parasite presented limited sensitivity, specificity and high cross-reactivity against other parasites, we identified 42, 58, 68 and 97-kDa semi-purified antigens as the most promising candidates for immunodiagnosis. Moreover, the 58 and 68-kDa antigens presented the lowest IgM cross-reactivity. When tested as a combination, a mixture of the 58 and 68-kDa antigens presented 100% sensitivity and specificity, as well as minor cross-reactivity. Although the combination of the 42, 58, 68 and 97-kDa antigens presented 100% sensitivity at a dilution of 1:40, the low specificity and high cross-reactivity observed suggested a limited use for diagnostic purposes. Our data suggested that the 58 and 68-kDa antigens might be most suitable for the immunodiagnosis of human toxocariasis. |
| Databáze: | Directory of Open Access Journals |
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