| Autor: |
Cheng-Chin Wang, Shi-Ying Huang, Shu-Hung Huang, Zhi-Hong Wen, Jyun-Ying Huang, Wen-Sheng Liu, Hui-Min David Wang |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Food Science and Human Wellness, Vol 6, Iss 4, Pp 195-201 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
2213-4530 |
| DOI: |
10.1016/j.fshw.2017.10.001 |
| Popis: |
The optimization of fermentation medium is important for synthetic biological secondary metabolite productions. The effect of rotation speed, inoculum amount, and medium supplements on the cell growth and Lycogen⢠secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was evaluated. The results reveal that a higher rotational speed exhibit a higher cell density, and the increasing in the amount of inoculum amount show a slight augment on the growth of R. sphaeroides WL-APD911.In the case of nitrogen sources adding, Lycogen⢠production was achieved with a 0.5 mM l-lysine supplementation. Moreover, the attention of Tween 80 presented a tremendous increase in the secondary metabolite. Response surface methodology (RSM) exhibited the optimization of medium supplements for Lycogen⢠invention is accomplished at molasses concentration of 10 g/L, yeast extract concentration of 40 g/L, 0.3% Tween 80 and NaCl concentration of 5 g/L, respectively. Further, the batch fermentation is carried out in both 5 L and 20 L fermentors to study the scale-up process factors to be adopted. At a 20 L fermentor, Lycogen⢠yields under the optimal culture condition are over 2 times than in the shake flask. The present results provide the Lycogen⢠optimal culture mediums, scale-up procedures and efficient extractions from R. sphaeroides WL-APD911. Keywords: Rhodobacter sphaeroides WL-APD911, Lycogenâ¢, Response surface methodology (RSM), Ferementation |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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