Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro

Autor: Isabel B. Lima-Verde, Maria H.T. Matos, Juliana J.H. Celestino, Rafael Rossetto, Khesller P.O. Name, Sônia N. Báo, Cláudio C. Campello, José R. Figueiredo
Jazyk: English<br />Portuguese
Rok vydání: 2012
Předmět:
Zdroj: Pesquisa Veterinária Brasileira, Vol 32, Iss 4, Pp 361-367 (2012)
Druh dokumentu: article
ISSN: 1678-5150
0100-736X
DOI: 10.1590/S0100-736X2012000400015
Popis: We investigated the effects of progesterone and follicle stimulating hormone (FSH) on survival and growth of caprine preantral follicles. Pieces of ovarian tissue were cultured for 1 or 7 days in minimum essential medium (MEM) alone or containing progesterone (1, 2.5, 5, 10 or 20ng/mL), FSH (50ng/mL) or the interaction between progesterone and FSH. Fresh (non-cultured control) and cultured ovarian tissues were processed for histological and ultrastructural studies. After 7 days the addition of FSH to all progesterone concentrations maintained the percentage of normal follicles similar to fresh control. At day 7 of culture, a higher percentage of developing follicles was observed only in 2.5ng/ml of progesterone associated with FSH or 10ng/ml of progesterone alone when compared with control. From day 1 to day 7 of culture, a significant increase in the percentage of developing follicles was observed in MEM and 2.5ng/ml of progesterone + FSH. In addition, after 7 days, in all treatments, there was a significant increase in follicular diameter when compared with control, except for MEM alone and in 5ng/ml of progesterone + FSH or 10ng/ml of progesterone alone. Ultrastructural studies confirmed follicular integrity after 7 days of culture in 2.5ng/ml of progesterone with FSH. In conclusion, this study demonstrated that the interaction between progesterone and FSH maintains ultrastructural integrity, stimulates primordial follicles activation and further growth of cultured caprine preantral follicles.
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