Interferon-beta enhances sensitivity to gemcitabine in pancreatic cancer

Autor:	Amber Blaauboer, Stephanie Booy, Peter M. van Koetsveld, Bas Karels, Fadime Dogan, Suzanne van Zwienen, Casper H. J. van Eijck, Leo J. Hofland
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Pancreatic cancer Interferon-beta Gemcitabine Chemosensitising effect Drug transporters Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Zdroj:	BMC Cancer, Vol 20, Iss 1, Pp 1-15 (2020)
Druh dokumentu:	article
ISSN:	1471-2407
DOI:	10.1186/s12885-020-07420-0
Popis:	Abstract Background Adjuvant gemcitabine for pancreatic cancer has limited efficacy in the clinical setting. Impaired drug metabolism is associated with treatment resistance. We aimed to evaluate the chemosensitising effect of interferon-beta (IFN-β). Methods BxPC-3, CFPAC-1, and Panc-1 cells were pre-treated with IFN-β followed by gemcitabine monotherapy. The effect on cell growth, colony formation, and cell cycle was determined. RT-qPCR was used to measure gene expression. BxPC-3 cells were used in a heterotopic subcutaneous mouse model. Results IFN-β increased sensitivity to gemcitabine (4-, 7.7-, and 1.7-fold EC50 decrease in BxPC-3, CFPAC-1, and Panc-1, respectively; all P
Databáze:	Directory of Open Access Journals
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