| Popis: |
Endra Yustin Ellistasari,1 Harijono Kariosentono,2 Bambang Purwanto,3 Brian Wasita,4 Risya Cilmiaty Arief Riswiyant,5 Eti Poncorini Pamungkasari,6 Soetrisno Soetrisno7 1Doctoral Program of Medical Sciences Department, Sebelas Maret University, Surakarta, Indonesia; 2Dermatology and Venereology Department, Sebelas Maret University, Surakarta, Indonesia; 3Internal Medicine Department, Sebelas Maret University, Surakarta, Indonesia; 4Anatomical Pathology Department, Sebelas Maret University, Surakarta, Indonesia; 5Dentistry Department, Sebelas Maret University, Surakarta, Indonesia; 6Public Health Department, Sebelas Maret University, Surakarta, Indonesia; 7Obstetric and Gynecology Department, Sebelas Maret University, Surakarta, IndonesiaCorrespondence: Endra Yustin Ellistasari, Dermatology and Venereology Department, Dr Moewardi General Hospital/Faculty of Medicine Sebelas Maret University, Jl Kol Sutarto 132 Jebres, Surakarta, Central Java, Indonesia, Tel +62 271634634, Email endra_yustin@yahoo.comPurpose: This is an in-vitro experimental study to analyze the effect of Exo-HUVEC on endothelial cell (CD31), cell proliferation, matrix metalloproteinase 1 (MMP-1) and collagen type 1 on irradiated fibroblast with UVB as photo-aging model.Patients and Methods: Fibroblast cultures were divided into 5 groups, namely without UVB exposure, UVB exposure 600mJ/cm2 for 80 seconds as photo-aging model, and UVB exposure +Exo-HUVEC exposure 0.1%, 0.5% and 1%. The endothelial cell was stained with a CD31 marker, MMP-1 were examined with ELISA, cell proliferation is detected using an MTT assay; meanwhile, collagen type 1 deposition and endothelial cell were measured using flowcytometry.Results: This study found positive endothelial cell marker CD31. Significant difference was found in cell proliferation, MMP-1 and collagen type 1 level between the control group with UVB irradiation and the treatment group with Exo-HUVEC (p < 0.05).Conclusion: Exo-HUVEC significantly increases cell proliferation and collagen type 1 level, while decrease MMP-1 levels on irradiated fibroblast; therefore, Exo-HUVEC ameliorate the photo-aging of skin fibroblast.Graphical Abstract: Keywords: photo-aging, Exo-HUVEC, endothelial cell, cell proliferation, MMP-1, collagen type 1 |
