| Autor: |
Alfredo Gragnani, Bruno Rafael Müller, Ismael Dale Contrim Guerreiro da Silva, Samuel Marcos Ribeiro de Noronha, Lydia Masako Ferreira |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Acta Cirúrgica Brasileira, Vol 28, Iss 8, Pp 551-558 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
0102-8650 |
| DOI: |
10.1590/S0102-86502013000800001 |
| Popis: |
PURPOSE: To evaluate the gene expression of KGF, TNF-alpha and IL-1 beta in skin fibroblasts and keratinocytes cultured from burned patients. METHODS: Three patients with large burns and three patients with small burns, as well as two controls, were included. The cell culture was initiated by the enzymatic method. After extraction and purification of mRNA, qPCR was used to assess the gene expression of KGF, TNF-alpha and IL-1 beta. RESULTS: The expression of KGF was increased on average 220-fold in large burns and 33.33-fold in small burns in fibroblasts, and 11.2-fold in large burns and 3.45-fold in small burns in keratinocytes compared to healthy patients (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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