In Vitro and In Vivo Response of Zinc-Containing Mesoporous Bioactive Glasses in a Sheep Animal Model

Autor: Javier Jiménez-Holguín, Daniel Arcos, Daniel Lozano, Melchor Saiz-Pardo, David de Pablo, Luis Ortega, Silvia Enciso, Blanca Fernández-Tomé, Idoia Díaz-Güemes, Francisco Miguel Sánchez-Margallo, Laura Casarrubios, María Teresa Portolés, María Vallet-Regí
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: International Journal of Molecular Sciences, Vol 23, Iss 22, p 13918 (2022)
Druh dokumentu: article
ISSN: 1422-0067
1661-6596
97801429
DOI: 10.3390/ijms232213918
Popis: Zinc-enriched mesoporous bioactive glasses (MBGs) are bioceramics with potential antibacterial and osteogenic properties. However, few assays have been performed to study these properties in animal models. In this study, MBGs enriched with up to 5% ZnO were synthesized, physicochemically characterized, and evaluated for their osteogenic activity both in vitro and in vivo. The ZnO MBGs showed excellent textural properties despite ZnO incorporation. However, the release of Zn2+ ions inhibited the mineralization process when immersed in simulated body fluid. In vitro assays showed significantly higher values of viability and expression of early markers of cell differentiation and angiogenesis in a ZnO-content-dependent manner. The next step was to study the osteogenic potential in a sheep bone defect model. Despite their excellent textural properties and cellular response in vitro, the ZnO MBGs were not able to integrate into the bone tissue, which can be explained in terms of inhibition of the mineralization process caused by Zn2+ ions. This work highlights the need to develop nanostructured materials for bone regeneration that can mineralize to interact with bone tissue and induce the processes of implant acceptance, cell colonization by osteogenic cells, and regeneration of lost bone tissue.
Databáze: Directory of Open Access Journals
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