| Autor: |
PS Hansen, N Rüdiger, A Tybjaerg-Hansen, O Faergeman, N Gregersen |
| Jazyk: |
angličtina |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 32, Iss 7, Pp 1229-1233 (1991) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)41985-6 |
| Popis: |
A single primer-template mismatch 2 bp from the apoB-3500 (G to A) mutation permits introduction of a cleavage site for MspI (C/CGG) in normal alleles but not in mutant alleles (CCAG). After amplification, cleavage, and polyacrylamide gel electrophoresis, normal and mutant alleles could be unambiguously distinguished. We constructed a positive (homozygous mutant) standard by site-directed mutagenesis. A negative standard was DNA from a homozygous normal subject. The method enables us to screen for the mutation with 12 microliters of spotted whole blood as the source of DNA. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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