Time-series sewage metagenomics distinguishes seasonal, human-derived and environmental microbial communities potentially allowing source-attributed surveillance

Autor: Ágnes Becsei, Alessandro Fuschi, Saria Otani, Ravi Kant, Ilja Weinstein, Patricia Alba, József Stéger, Dávid Visontai, Christian Brinch, Miranda de Graaf, Claudia M. E. Schapendonk, Antonio Battisti, Alessandra De Cesare, Chiara Oliveri, Fulvia Troja, Tarja Sironen, Olli Vapalahti, Frédérique Pasquali, Krisztián Bányai, Magdolna Makó, Péter Pollner, Alessandra Merlotti, Marion Koopmans, Istvan Csabai, Daniel Remondini, Frank M. Aarestrup, Patrick Munk
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Nature Communications, Vol 15, Iss 1, Pp 1-13 (2024)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-024-51957-8
Popis: Abstract Sewage metagenomics has risen to prominence in urban population surveillance of pathogens and antimicrobial resistance (AMR). Unknown species with similarity to known genomes cause database bias in reference-based metagenomics. To improve surveillance, we seek to recover sewage genomes and develop a quantification and correlation workflow for these genomes and AMR over time. We use longitudinal sewage sampling in seven treatment plants from five major European cities to explore the utility of catch-all sequencing of these population-level samples. Using metagenomic assembly methods, we recover 2332 metagenome-assembled genomes (MAGs) from prokaryotic species, 1334 of which were previously undescribed. These genomes account for ~69% of sequenced DNA and provide insight into sewage microbial dynamics. Rotterdam (Netherlands) and Copenhagen (Denmark) show strong seasonal microbial community shifts, while Bologna, Rome, (Italy) and Budapest (Hungary) have occasional blooms of Pseudomonas-dominated communities, accounting for up to ~95% of sample DNA. Seasonal shifts and blooms present challenges for effective sewage surveillance. We find that bacteria of known shared origin, like human gut microbiota, form communities, suggesting the potential for source-attributing novel species and their ARGs through network community analysis. This could significantly improve AMR tracking in urban environments.
Databáze: Directory of Open Access Journals
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