Isolation of Mitochondria From Fresh Mice Lung Tissue
Autor: | Dayene de Assis Fernandes Caldeira, Dahienne Ferreira de Oliveira, João Paulo Cavalcanti-de-Albuquerque, Jose Hamilton Matheus Nascimento, Walter Araujo Zin, Leonardo Maciel |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: | |
Zdroj: | Frontiers in Physiology, Vol 12 (2021) |
Druh dokumentu: | article |
ISSN: | 1664-042X |
DOI: | 10.3389/fphys.2021.748261 |
Popis: | Direct analysis of isolated mitochondria enables a better understanding of lung dysfunction. Despite well-defined mitochondrial isolation protocols applicable to other tissues, such as the brain, kidney, heart, and liver, a robust and reproductive protocol has not yet been advanced for the lung. We describe a protocol for the isolation of mitochondria from lung tissue aiming for functional analyses of mitochondrial O2 consumption, transmembrane potential, reactive oxygen species (ROS) formation, ATP production, and swelling. We compared our protocol to that used for heart mitochondrial function that is well-established in the literature, and achieved similar results. |
Databáze: | Directory of Open Access Journals |
Externí odkaz: |
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