Isolation of Mitochondria From Fresh Mice Lung Tissue

Autor: Dayene de Assis Fernandes Caldeira, Dahienne Ferreira de Oliveira, João Paulo Cavalcanti-de-Albuquerque, Jose Hamilton Matheus Nascimento, Walter Araujo Zin, Leonardo Maciel
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Frontiers in Physiology, Vol 12 (2021)
Druh dokumentu: article
ISSN: 1664-042X
DOI: 10.3389/fphys.2021.748261
Popis: Direct analysis of isolated mitochondria enables a better understanding of lung dysfunction. Despite well-defined mitochondrial isolation protocols applicable to other tissues, such as the brain, kidney, heart, and liver, a robust and reproductive protocol has not yet been advanced for the lung. We describe a protocol for the isolation of mitochondria from lung tissue aiming for functional analyses of mitochondrial O2 consumption, transmembrane potential, reactive oxygen species (ROS) formation, ATP production, and swelling. We compared our protocol to that used for heart mitochondrial function that is well-established in the literature, and achieved similar results.
Databáze: Directory of Open Access Journals
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