Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview

Autor: Giulia Sabbatinelli, Donatella Fantasia, Chiara Palka, Elisena Morizio, Melissa Alfonsi, Giuseppe Calabrese
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Diagnostics, Vol 11, Iss 12, p 2239 (2021)
Druh dokumentu: article
ISSN: 2075-4418
DOI: 10.3390/diagnostics11122239
Popis: Prenatal diagnosis plays a crucial role in clinical genetics. Non-invasive prenatal diagnosis using fetal cells circulating in maternal peripheral blood has become the goal of prenatal diagnosis, to obtain complete fetal genetic information and avoid risks to mother and fetus. The development of high-efficiency separation technologies is necessary to obtain the scarce fetal cells from the maternal circulation. Over the years, multiple approaches have been applied, including choice of the ideal cell targets, different cell recovering technologies, and refined cell isolation yield procedures. In order to provide a useful tool and to give insights about limitations and advantages of the technologies available today, we review the genetic research on the creation and validation of non-invasive prenatal diagnostic testing protocols based on the rare and labile circulating fetal cells during pregnancy.
Databáze: Directory of Open Access Journals
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