Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke
| Autor: | Conita Yuniarifa, Joko Wahyu Wibowo, Taufiqurrachman Nasihun |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Sains Medika, Vol 11, Iss 2, Pp 81-88 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2085-1545 2339-093X |
| DOI: | 10.30659/sainsmed.v11i2.2465 |
| Popis: | Introduction: The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility. Objective: to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli and Leydig cells, sperm quality and texpression of caspase-3 in rats exposed to cigarette smoke. Methods: This was an experimental study using a post test only control group design. Thirty six rats were randomly divided into 6 groups to receive one of the following combinations of antioxidants: vit C and E, glutathione, zinc (T4-G); vit C and E, glutathione (T3-G); vit C and vit E (T2-G); Glutathione, zinc (T1-G); control positive (CP-G) cigarette smoke exposed only, normal (CN-G). The dose of vit C, vit E, glutathion and zinc was 9 mg/day, 1.8 IU/day, 1.8 mg/day, 0.2 mg/day respectively. All groups were exposed to cigarette smoke (3 cigarettes/day for 21 days), except for the CN-G group. On day 22, sperm samples were taken. Testicular tissue was taken for measurements of sperm quality, sertoli and leydig cell.numbers. Results: There was a significant difference in mean number of Leydig cells, sperm motility, sperm count and caspase-3 between groups (p |
| Databáze: | Directory of Open Access Journals |
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