Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling
| Autor: | Chih-Pin Chuu, Ya-Wen Chen, Horng-Dar Wang, Jang-Yang Chang, Hung-Che Chiang, Chi-Jung Chung, Ping-Hsuan Hsiao, Jonathan Yang, Liang-Cheng Su, Ying-Yu Kuo, Chieh Huo, Hui-Ping Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
oral cancer
caffeic acid phenethyl ester TW2.6 cell proliferation cell cycle Akt Akt1 Akt2 phospho-Akt Ser473 phospho-Akt Thr 308 FOXO1 FOXO3a phospho-FOXO1 Thr24 phospho-FoxO3a Thr32 NF-κB phospho-NF-κB Ser536 Rb phospho-Rb Ser807/811 Skp2 cyclin D1 p27 5-fluorouracil Biology (General) QH301-705.5 Chemistry QD1-999 |
| Zdroj: | International Journal of Molecular Sciences, Vol 14, Iss 5, Pp 8801-8817 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms14058801 |
| Popis: | Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1 phase cell population, increased G2/M phase cell population, and induced apoptosis in TW2.6 cells. Treatment with CAPE decreased protein abundance of Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr 308, GSK3β, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FoxO3a Thr32, NF-κB, phospho-NF-κB Ser536, Rb, phospho-Rb Ser807/811, Skp2, and cyclin D1, but increased cell cycle inhibitor p27Kip. Overexpression of Akt1 or Akt2 in TW2.6 cells rescued growth inhibition caused by CAPE treatment. Co-treating TW2.6 cells with CAPE and 5-fluorouracil, a commonly used chemotherapeutic drug for oral cancers, exhibited additive cell proliferation inhibition. Our study suggested that administration of CAPE is a potential adjuvant therapy for patients with OSCC oral cancer. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|