Oxidative damage in Echinochloa crus−galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination

Autor: Naphat Somala, Nutcha Manichart, Chamroon Laosinwattana, Pattharin Wichittrakarn, Kaori Yoneyama, Montinee Teerarak, Nawasit Chotsaeng
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Frontiers in Agronomy, Vol 6 (2024)
Druh dokumentu: article
ISSN: 2673-3218
DOI: 10.3389/fagro.2024.1456168
Popis: This study investigates the inhibitory effects of Diaporthe sp. isolate EC010 extract on barnyardgrass (Echinochloa crus-galli) seed germination and growth. Application of sequential extraction techniques to Diaporthe sp. mycelium resulted in partial separation of the phytotoxic compounds. The ethyl acetate (EtOAc) fraction most greatly reduced seed germination (81.01%), root length (89.18%), and shoot length (84.74%) compared to the control. Chemical characterization using gas chromatography-mass spectrometry revealed major constituents of linoleic acid, butyl ester (9.69%), hexadecanoic acid (7.99%), and 14-pentadecenoic acid (7.86%). With regard to physiological and biochemical indexes, treated seeds exhibited lower imbibition, significantly decreased α-amylase (EC 3.2.1.1) activity (p
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