| Autor: |
Maria Elena Melica, Maria Lucia Angelotti, Giulia Antonelli, Anna Peired, Carolina Conte, Letizia De Chiara, Benedetta Mazzinghi, Elena Lazzeri, Laura Lasagni, Paola Romagnani |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Bio-Protocol, Vol 13, Iss 16 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2331-8325 |
| DOI: |
10.21769/BioProtoc.4757 |
| Popis: |
Kidney diseases are a global health concern. Modeling of kidney disease for translational research is often challenging because of species specificities or the postmitotic status of kidney epithelial cells that make primary cultures, for example podocytes. Here, we report a protocol for preparing primary cultures of podocytes based on the isolation and in vitro propagation of immature kidney progenitor cells subsequently differentiated into mature podocytes. This protocol can be useful for studying physiology and pathophysiology of human kidney progenitors and to obtain differentiated podocytes for modeling podocytopathies and other kidney disorders involving podocytes.Graphical overview |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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