| Autor: |
Thi-Thuy-Anh Nguyen, Thanh-Hao Do, Thi-Thu-Hien Pham, Ngoc-Trinh Huynh |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of HerbMed Pharmacology, Vol 11, Iss 1, Pp 48-54 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2345-5004 |
| DOI: |
10.34172/jhp.2022.05 |
| Popis: |
Introduction: Perilla frutescens (L.) Britt leaves contain various phenolic compounds, especially flavonoids, which potentially inhibit tyrosinase, the key enzyme catalyzing the melanin synthesis pathway. This work aimed to investigate the anti-melanogenic effect of Perilla frutescens extracts through the inhibition of non-cellular as well as cellular tyrosinase activities. Methods: The total extract from Perilla frutescens leaves was obtained by percolation method with 50% ethanol followed by liquid–liquid partition with ethyl acetate (EA) and chloroform (CF) to obtain the EA, CF and EA/CF extracts. The mushroom tyrosinase inhibitory effect of the obtained extracts was screened by dopachrome formation reactions from its substrate 3,4-dihydroxy-L-phenylalanine. The extracts with potential activity were further evaluated for the anti-melanogenic effects in the B16F10 melanoma cell line. Results: EA and EA/CF extracts significantly inhibited mushroom tyrosinase activity with the IC50s of 0.14 and 0.07 mg/mL, respectively, in the same range of that from kojic acid (0.12 mg/mL). Consistently, in B16F10 cells, these extracts inhibited tyrosinase enzyme; their IC50 values were 2 times lower than that of kojic acid. Moreover, both EA and EA/CF extracts remarkably reduced melanin levels in a concentration-dependent manner. Conclusion: The EA and EA/CF extracts from Perilla frutescens leaves were able to inhibit melanogenesis by reducing tyrosinase activity. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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