| Autor: |
Poonam Sikka, Kunwar Pal Singh, Inderjeet Singh, Dwijesh Chandra Mishra, Shyam Sundar Paul, Ashok K. Balhara, Jerome Andonissamy, Krishna Kumar Chaturvedi, Atmakuri Ramakrishna Rao, Anil Rai |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Frontiers in Animal Science, Vol 4 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2673-6225 |
| DOI: |
10.3389/fanim.2023.1135429 |
| Popis: |
Functional genome profiling of Murrah buffaloes (Bubalus bubalis) was performed for milk-production trait by whole blood transcriptome analysis comparing RNA-seq data assembled from high and low milk producing multiparous (5 -6 parity) animals. These buffaloes reflected the genetic merit inherited as daughters born to extremely high- and low-end bulls evaluated under a progeny testing scheme and ranked by the estimated breeding value. The average standard milk yield (SMY) over the 305 d during the parity was recorded as 2909.50L ± 492.63 and 1869.57 ± 189.36L in high- and low-performance buffaloes, respectively. The “reference” assembly data was assembled from transcriptome libraries of a group of buffaloes (n=16), comprising of animals in different physiological states. Replicates selected within each category of the high and low genetic merit animals showed a correlation coefficient of high order (R2=0.98) while comparing with the `reference' assembly. The sequence data of selected buffaloes, mapped over the Mediterranean water buffalo genome, revealed differentially expressed genes (DEGs) distinctly depicted via heat maps and volcano plots obtained for two categories of animals, determining more than 25,000 genes via the Cufflink analysis. DEGs included 83 down-regulating and 142 up-regulating genes (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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