| Autor: |
Fuminobu Sato, Kikuo Shimizu, Isao Murata |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Journal of Radiation Research and Applied Sciences, Vol 11, Iss 1, Pp 83-88 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
1687-8507 |
| DOI: |
10.1016/j.jrras.2017.11.001 |
| Popis: |
A single floating cell irradiation technique with X-ray microbeam has been developed for the microscopic research of radiation effects on floating cells in a liquid medium. This technique is a combination of X-ray microbeam and laser technologies. A preliminary experiment on the survival of budding cells of the yeast Saccharomyces cerevisiae (RAD52) was performed with the X-ray microbeam system. Micro-pits were fabricated on the bottom of a culture dish in a liquid medium, using green laser beams. The yeast cells were put into the micro-pits one by one by using focused infrared laser beams. A 50-kV X-ray microbeam 12 μm (FWHM) in diameter was propagated into the targeted yeast cell. The maximum dose rate on the targeted cell was estimated to be 0.09 Gy/s from the results of beam profile measurements and photon-electron transport calculations. The X-ray irradiation effect on the cell lethality was clearly observed for the cell exposed to X-rays of 100 Gy. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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