| Autor: |
Lisandra Serra Damasceno, Tania Vite-Garín, José Antonio Ramírez, Gabriela Rodríguez-Arellanes, Marcos Abreu de Almeida, Mauro de Medeiros Muniz, Jacó Ricarte Lima de Mesquita, Terezinha do Menino Jesus Silva Leitão, Maria Lucia Taylor, Rosely Maria Zancopé-Oliveira |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 61, Iss 0 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1678-9946 |
| DOI: |
10.1590/s1678-9946201961008 |
| Popis: |
ABSTRACT Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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