| Autor: |
Pradeep Chaluvally-Raghavan, Kang Jin Jeong, Sunila Pradeep, Andreia Machado Silva, Shuangxing Yu, Wenbin Liu, Tyler Moss, Cristian Rodriguez-Aguayo, Dong Zhang, Prahlad Ram, Jinsong Liu, Yiling Lu, Gabriel Lopez-Berestein, George A. Calin, Anil K. Sood, Gordon B. Mills |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Cell Reports, Vol 15, Iss 7, Pp 1493-1504 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2211-1247 |
| DOI: |
10.1016/j.celrep.2016.04.034 |
| Popis: |
Summary: 3q26.2 amplification in high-grade serous ovarian cancer leads to increased expression of mature microRNA miR551b-3p, which is associated with poor clinical outcome. Importantly, miR551b-3p contributes to resistance to apoptosis and increased survival and proliferation of cancer cells in vitro and in vivo. miR551b-3p upregulates STAT3 protein levels, and STAT3 is required for the effects of miR551b-3p on cell proliferation. Rather than decreasing levels of target mRNA as expected, we demonstrate that miR551b-3p binds a complementary sequence on the STAT3 promoter, recruiting RNA polymerase II and the TWIST1 transcription factor to activate STAT3 transcription, and thus directly upregulates STAT3 expression. Furthermore, anti-miR551b reduced STAT3 expression in ovarian cancer cells in vitro and in vivo and reduced ovarian cancer growth in vivo. Together, our data demonstrate a role for miR551b-3p in transcriptional activation. Thus, miR551b-3p represents a promising candidate biomarker and therapeutic target in ovarian cancer. : Chaluvally-Raghavan et al. find that miR551b-3p affects transcription of STAT3. The authors find that miR551b-3p binds to the STAT3 promoter and facilitates RNA polymerase II and TWIST1 transcription factor recruitment, which in turn activates STAT3 transcription. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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