Suppressive effects of (-)-tubaic acid on RANKL-induced osteoclast differentiation and bone resorption

Autor: Soomin Lim, Hye Jung Ihn, Ju Ang Kim, Jong-Sup Bae, Jung-Eun Kim, Yong Chul Bae, Hong-In Shin, Tae Hoon Kim, Eui Kyun Park
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Animal Cells and Systems, Vol 27, Iss 1, Pp 1-9 (2023)
Druh dokumentu: article
ISSN: 19768354
2151-2485
1976-8354
DOI: 10.1080/19768354.2023.2166107
Popis: ABSTRACTRegulation of osteoclastogenesis and bone-resorbing activity can be an efficacious strategy for treating bone loss diseases because excessive osteoclastic bone resorption leads to the development of such diseases. Here, we investigated the role of (-)-tubaic acid, a thermal degradation product of rotenone, in osteoclast formation and function in an attempt to identify alternative natural compounds. (-)-Tubaic acid significantly inhibited receptor activator of nuclear factor-κB ligand (RANKL)-mediated osteoclast differentiation at both the early and late stages, suggesting that (-)-tubaic acid affects the commitment and differentiation of osteoclast progenitors as well as the cell-cell fusion of mononuclear osteoclasts. (-)-Tubaic acid attenuated the activation of extracellular signal-regulated kinase (ERK) and expression of nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) and its target genes in response to RANKL. Furthermore, a pit-formation assay revealed that (-)-tubaic acid significantly impaired the bone-resorbing activity of osteoclasts. Our results demonstrated that (-)-tubaic acid exhibits anti-osteoclastogenic and anti-resorptive effects, indicating its therapeutic potential in the management of osteoclast-related bone diseases.
Databáze: Directory of Open Access Journals
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