| Autor: |
Joseph Stocks, M. Nazeem Nanjee, Norman E. Miller |
| Jazyk: |
angličtina |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 39, Iss 1, Pp 218-227 (1998) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)34217-6 |
| Popis: |
We describe new methods for analyzing the apolipoproteins (apo) of the high density lipoproteins (HDL) of several species by two modes of capillary electrophoresis: size separation using a molecular sieving buffer, and capillary zone electrophoresis (CZE) using neutral coated capillaries. By either mode HDL apos were resolved within 25 min. Results for apoA-I and apoA-II mass agreed with those by electroimmunoassay; intra-assay coefficients of variation were 1.8–4.2%. The migration times of human, rat, rabbit, and bovine apoA-I during CZE were proportional to their net charge/Mr ratios. This enabled human and rabbit apoA-I to be quantified simultaneously in transgenic rabbit HDLs. CZE also resolved human apoA-I isoforms, deamidated apoA-I, and pro-apoA-I.—Stocks, J., M. N. Nanjee, and N. E. Miller. Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis. J. Lipid Res. 1998. 39: 218–227. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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