Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols

Autor: R. Baeza-Jiménez, K. Miranda, H. S. García, C. Otero
Jazyk: English<br />Spanish; Castilian
Rok vydání: 2013
Předmět:
Zdroj: Grasas y Aceites, Vol 64, Iss 3, Pp 237-242 (2013)
Druh dokumentu: article
ISSN: 0017-3495
1988-4214
DOI: 10.3989/gya.084412
Popis: Diacylglycerols (DAG) rich in n-3 residues were successfully produced by Novozym 435-catalysed glycerolysis of n-3 PUFA rich fish oil. Orbital and magnetic agitations were evaluated in order to minimize mass transfer limitations and thus assure the homogeneity of the reactant mixture. Different temperatures (65, 70, 75, 80, 85 and 90 °C) and speeds (300, 500, 700 and 900 rpm) were tested. Optimal conditions to obtain the highest amount of DAG were: 65 °C, with a substrate molar ratio of 3:1 (oil:glycerol), 500 rpm and 15% enzyme load after 2.5 h, with a yield of 60%.
Databáze: Directory of Open Access Journals