| Autor: |
Azam Torabi, Mojtaba Tahmoorespour, Fatemeh Vahedi, Nader Mosavari, Mohammadreza Nassiri |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Reports of Biochemistry and Molecular Biology, Vol 2, Iss 1, Pp 35-41 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
2322-3480 |
| Popis: |
Background: Mycobacterium (M.) bovis is the agent of bovine tuberculosis (TB) in a range of animal species, including humans. Recent advances in immunology and the molecular biology of Mycobacterium have allowed identification of a large number of antigens with the potential for the development of a new TB vaccine. The ESAT-6 and CFP-10 proteins of M. bovis are important structural and functional proteins known to be important immunogens. Methods: In the current study, the DNAs encoding these genes were utilized in the construction of pcDNA 3.1+/ESAT-6 and pcDNA3.1+/CFP-10 plasmids. After intramuscular injection of BALB/c mice with these plasmids, ESAT-6 and CFP-10 mRNA expression was assessed by RT-PCR. Mice were inoculated and boosted with the plasmids to evaluate their effects on lymphocyte proliferation. Results: Our results indicate the plasmids are expressed at the RNA level and can induce lymphocyte proliferation. Conclusion: Further study is needed to characterize the effect of these antigens on the immune system and determine whether they are effective vaccine candidates against M. bovis. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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