Quantitative analysis of collagen morphology in breast cancer from millimeter scale using multiphoton microscopy

Autor: Yulan Liu, Shunwu Xu, Deyong Kang, Xingxin Huang, Shuoyu Xu, Lianhuang Li, Liqin Zheng, Lida Qiu, Zhenlin Zhan, Xiahui Han, Jianxin Chen
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Journal of Innovative Optical Health Sciences, Vol 16, Iss 04 (2023)
Druh dokumentu: article
ISSN: 17935458
1793-7205
1793-5458
DOI: 10.1142/S1793545822430039
Popis: The tumor microenvironment (TME) is now recognized as an important participant of tumor progression. As the most abundant extracellular matrix component in TME, collagen plays an important role in tumor development. The imaging study of collagen morphological feature in TME is of great significance for understanding the state of tumor. Multiphoton microscopy (MPM), based on second harmonic generation (SHG) and two-photon excitation fluorescence (TPEF), can be used to monitor the morphological changes of biological tissues without labeling. In this study, we used MPM for large-scale imaging of early invasive breast cancer from the tumor center to normal tissues far from the tumor. We found that there were significant differences in collagen morphology between breast cancer tumor boundary, near tumor transition region and normal tissues far from the tumor. Furthermore, the morphological feature of eight collagen fibers was extracted to quantify the variation trend of collagen in three regions. These results may provide a new perspective for the optimal negative margin width of breast-conserving surgery and the understanding of tumor metastasis.
Databáze: Directory of Open Access Journals
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