The Modulatory Effects of Vitamin D on the Expression of IL-12 and TGF-β in the Spinal Cord and Serum of Mice with Experimental Autoimmune Encephalomyelitis
| Autor: | Rayhaneh Ahangar-Parvin, Marzieyeh Mohammadi-Kordkhayli, Sayyed Vahab Azizi, Maryam Nemati, Hossian Khorramdel-Azad, Zahra Taghipour, Zuhair Hassan, Seyed Mohammad Moazzeni, Abdollah Jafarzadeh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Iranian Journal of Pathology, Vol 13, Iss 1, Pp 10-22 (2018) |
| Druh dokumentu: | article |
| ISSN: | 1735-5303 2345-3656 |
| DOI: | 10.30699/ijp.13.1.10 |
| Popis: | Background & objective The immunoregulatory effects of transforming growth factor (TGF)-βand interleukin-12 (IL-12) and immunomodulatory actions of vitamin D (VD) were reported in several studies. This study aims to evaluate VD effects on IL-12 and TGF-β expression in experimental autoimmune encephalomyelitis (EAE). Methods: EAE was induced in three groups of C57BL/6 mice by immunization with MOG and administered intra-peritoneally 200 ngVD, PBS or olive oil (OO) from day +3 to +30. One group was also considered as healthy control group. At day 31, cytokines expression in the spinal cord and their serum levels were determined using real time-PCR and ELISA, respectively. Results: IL-12 gene expression and its serum levels in PBS-injected- or OO-administrated EAE groups were significantly higher than healthy group. IL-12 gene expression in EAE group treated with VD was significantly decreased compared to PBS-injected- or OO-administrated EAE groups (P Conclusion: VD modulates the expression of IL-12 and TGF-β in spinal cord and serum of EAE mice. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|