Allele Specific Polymerase Chain Reaction (AS-PCR) Assay to Detect Knockdown and Acetylcholinesterase Mutations in Anopheles superpictus
| Autor: | Sare İlknur Yavaşoğlu, Fatih Mehmet Şimşek |
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| Jazyk: | English<br />Turkish |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Türkiye Parazitoloji Dergisi, Vol 46, Iss 4, Pp 307-311 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1300-6320 2146-3077 |
| DOI: | 10.4274/tpd.galenos.2022.20592 |
| Popis: | Objective:The study aims to determine the presence of L1014F, L1014S, L1014C alleles, which are responsible for knockdown resistance and Ace-1 G119S alleles, which are responsible for acetylcholinesterase insensitivity in Anopheles superpictus, the secondary vector of malaria in Turkey.Methods:In this study, 60 Anopheles superpictus adult females were collected from Aydın, Denizli, and Muğla provinces. Then, allele-specific primers for kdr L1014F, L1014S, and L1014C alleles, and the Ace-1 G119S allele were designed. The presence of these alleles was screened in three Anopheles superpictus populations by allele-specific polymerase chain reaction.Results:Although L1014S allele frequency was too low in Aydın, Muğla, and Denizli populations, neither kdr L1014F and L1014C nor Ace-1 G119S mutations were found in any population.Conclusion:In this study, kdr L1014S mutation was detected for the first time in the Aegean Anopheles superpictus populations. |
| Databáze: | Directory of Open Access Journals |
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