Antitumor, toxicity and target gene expression evaluation of MiR-204-5p mimic application on melanoma b16-bearing mice
Autor: | E. Z. Lapkina, N. V. Palkinа, A. S. Averchuk, A. R. Esimbekova, T. G. Ruksha |
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Jazyk: | ruština |
Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Сибирский онкологический журнал, Vol 21, Iss 3, Pp 61-69 (2022) |
Druh dokumentu: | article |
ISSN: | 1814-4861 2312-3168 |
DOI: | 10.21294/1814-4861-2022-21-3-61-69 |
Popis: | Objective. To evaluate anti-tumor, toxic effect of miR-204-5p mimic applicaton on melanoma B-16-bearing mice followed by miR-204-5p target gene expression estimation in melanoma tumor and distant organs. Material and Methods. C57Bl/6 melanoma B-16-bearing mice were used. The animals of the experimental group were intraperitoneally injected with a 5 nM miR-204-5p miRNA simulator (mimic) on the 8th, 10th, and 12th days after melanoma B-16 cell transplantation. Based on the results of bioinformatic analysis, miR-204-5p target genes BCL2 and SIRT1 expression levels were determined by quantitative real-time PCR. The toxic effect of miR-204-5p mimic was estimated by the evaluation of body weight, mass of the internal organs, and motor activity. Results. On the 13-14th days of the experiment, the motor activity of animals in the control groups decreased signifcantly compared to the group of animals treated by miR-204-5p. Target gene BCL2 showed increased expression in the lungs and kidneys and SIRT1 levels were increased in the lungs of miR-204-5p mimic treated animals (p˂0.05). Tumor mass tended to decrease in the animals treated by miR-204-5p mimic. Conclusion. Modulation of the level of miR-204-5p microRNA led to changes in the expression of SIRT1 and BCL2 in the lungs of animals, and changes in the expression of BCL2in the kidneys. MiR-204-5p mimic application did not have toxic effect on animals treated. Further studies are necessary to clarify miR-204-5p implication in melanoma cell proliferation regulation as well as it’s biodistibution in the tumor tissue. |
Databáze: | Directory of Open Access Journals |
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