| Autor: |
James J. Fabrizio, Janet Rollins, Christopher W. Bazinet, Stephanie Wegener, Iryna Koziy, Rachel Daniel, Vincent Lombardo, Dwaine Pryce, Kavita Bharrat, Elissa Innabi, Marielle Villanobos, Gabriela Mendoza, Elisa Ferrara, Stephanie Rodway, Matthew Vicioso, Victoria Siracusa, Erin Dailey, Justin Pronovost, Simon Innabi, Vrutant Patel, Nicole DeSouza, Danielle Quaranto, Amir Niknejad |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Biology Open, Vol 9, Iss 2 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2046-6390 |
| DOI: |
10.1242/bio.049080 |
| Popis: |
Individual sperm cells are resolved from a syncytium during late step of spermiogenesis known as individualization, which is accomplished by an Individualization Complex (IC) composed of 64 investment cones. mulet encodes Tubulin-binding cofactor E-like (TBCEL), suggesting a role for microtubule dynamics in individualization. Indeed, a population of ∼100 cytoplasmic microtubules fails to disappear in mulet mutant testes during spermatogenesis. This persistence, detected using epi-fluorescence and electron microscopy, suggests that removal of these microtubules by TBCEL is a prerequisite for individualization. Immunofluorescence reveals TBCEL expression in elongated spermatid cysts. In addition, testes from mulet mutant males were rescued to wild type using tubulin-Gal4 to drive TBCEL expression, indicating that the mutant phenotype is caused by the lack of TBCEL. Finally, RNAi driven by bam-GAL4 successfully phenocopied mulet, confirming that mulet is required in the germline for individualization. We propose a model in which the cytoplasmic microtubules serve as alternate tracks for investment cones in mulet mutant testes. This article has an associated First Person interview with the first author of the paper. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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