Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis

Autor: Lulin Huang, Lin Ye, Runze Li, Shanshan Zhang, Chao Qu, Shujin Li, Jie Li, Mu Yang, Biao Wu, Ran Chen, Guo Huang, Bo Gong, Zheng Li, Hongjie Yang, Man Yu, Yi Shi, Changguan Wang, Wei Chen, Zhenglin Yang
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Genes and Diseases, Vol 10, Iss 6, Pp 2540-2556 (2023)
Druh dokumentu: article
ISSN: 2352-3042
DOI: 10.1016/j.gendis.2022.11.007
Popis: The retinal pigment epithelium (RPE) and choroid are located behind the human retina and have multiple functions in the human visual system. Knowledge of the RPE and choroid cells and their gene expression profiles are fundamental for understanding retinal disease mechanisms and therapeutic strategies. Here, we sequenced the RNA of about 0.3 million single cells from human RPE and choroids across two regions and seven ages, revealing regional and age differences within the human RPE and choroid. Cell–cell interactions highlight the broad connectivity networks between the RPE and different choroid cell types. Moreover, the transcription factors and their target genes change during aging. The coding of somatic variations increases during aging in the human RPE and choroid at the single-cell level. Moreover, we identified ELN as a candidate for improving RPE degeneration and choroidal structure during aging. The mapping of the molecular architecture of the human RPE and choroid improves our understanding of the human vision support system and offers potential insights into the intervention targets for retinal diseases.
Databáze: Directory of Open Access Journals