| Autor: |
Mashhoor Kattali, Keerthana P. Mampett, Hamna Fathima Kodoor, Sreejesh Govindankutty Ponnenkunnathu, Somy Soman, Debarshi Kar Mahapatra, Tomy Muringayil Joseph, Józef Haponiuk, Sabu Thomas |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Eng, Vol 5, Iss 2, Pp 600-613 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2673-4117 |
| DOI: |
10.3390/eng5020034 |
| Popis: |
Microorganisms (MOs) are prominent in ecological functioning and balance. The rhizosphere is considered one of the most diverse ecosystems on Earth and serves as a breeding spot for many MOs. Rhizosphere microbial diversity changes according to plant species, genotype, and the nature of the soil. The current study reports the possible use of bacteria isolated from the rhizosphere of Azadirachta indica for synthesizing silver nanoparticles (AgNPs). The physicochemical characterization and antibacterial activity of these green synthesized AgNPs are also reported. The gene (16S rRNA) sequence of bacteria isolated from the rhizosphere showed a maximum similarity of 99.25% with Bacillus subtilis. After incubation, the colorless reaction mixture transformed to brown, which indicates the formation of AgNPs, and UV-vis spectral analysis also confirmed the biosynthesis of AgNPs. Compared to lower temperatures, the efficiency of AgNP synthesis was high at the higher temperature. The scanning electron microscope image demonstrated spherical-shaped AgNPs with sizes ranging from 18 to 21 nm. Energy-dispersive X-ray analysis established the elemental analysis of synthesized AgNPs. The synthesized AgNPs showed strong bactericidal properties against pathogenic bacteria Klebsiella pneumonia, Pseudomonas aeruginosa, Escherichia coli, and methicillin-resistant Staphylococcus aureus. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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