| Autor: |
Eleonora Fusi, Raffaella Rebucci, Chiara Pecorini, Anna Campagnoli, Luciano Pinotti, Francesca Saccone, Federica Cheli, Stig Purup, Kristen Sejrsen, Antonella Baldi |
| Jazyk: |
angličtina |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Toxins, Vol 2, Iss 6, Pp 1265-1278 (2010) |
| Druh dokumentu: |
article |
| ISSN: |
2072-6651 |
| DOI: |
10.3390/toxins2061265 |
| Popis: |
The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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