Diagnosis and Characterization of Leishmania Species in Patients and Rodents Giemsa-Stained Slides by PCR-RFLP in Damghan District, Iran
| Autor: | Sadegh Mohammadi Azni, Yavar Rasi, Mohammad Ali Oshaghi, Mohammadreza Yaghoubi Ershadi, Mahdi Mohebali, Mohammadreza Abaie, Homa Hajaran, Zeynab Nokandeh |
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| Jazyk: | perština |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | پزشکی بالینی ابن سینا, Vol 17, Iss 4, Pp 5-9 (2011) |
| Druh dokumentu: | article |
| ISSN: | 2588-722X 2588-7238 |
| Popis: | Introduction & Objective: Leishmaniasis is one of the most important infectious diseases in the world. This disease is reported from more than 15 provinces of Iran as a health problem. Identification of reservoirs and characterization of parasite species in patients is very important for identifying the epidemiological aspects of disease and planning for control program. Materials & Methods: This survey was performed as a descriptive study by PCR-RFLP method in Damghan district. Study was carried out on giemsa-stained smears from cases of leishmaniasis and rodents which collected in infected villages that leishmanal infection was confirmed by microscopic observations. DNA of giemsa-stained slides was extracted and followed by conventional PCR technique using specific primers of ITS1. PCR products were digested with a restriction enzyme (HaeIII). Results: Totally 25 smears from human skin lesions and 8 rodent ear scrapings were examined by PCR. Electrophoresis results showed that leishmania major was present in patients and rodents giemsa-stained slides. Conclusion: PCR-RFLP is an effective method to identify leishmania species from giemsa-stained smears which have been collected from humans and animal reservoirs. Using this method identification of causative species of leishmaniasis is possible without sequencing. |
| Databáze: | Directory of Open Access Journals |
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