Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins

Autor:	Indra D. Sahu, Gary A. Lorigan
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	membrane protein electron paramagnetic resonance (EPR) site-directed spin labeling structural and dynamics membrane mimetic double electron electron resonance (DEER) Microbiology QR1-502
Zdroj:	Biomolecules, Vol 10, Iss 5, p 763 (2020)
Druh dokumentu:	article
ISSN:	2218-273X
DOI:	10.3390/biom10050763
Popis:	Membrane proteins possess a variety of functions essential to the survival of organisms. However, due to their inherent hydrophobic nature, it is extremely difficult to probe the structure and dynamic properties of membrane proteins using traditional biophysical techniques, particularly in their native environments. Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a very powerful and rapidly growing biophysical technique to study pertinent structural and dynamic properties of membrane proteins with no size restrictions. In this review, we will briefly discuss the most commonly used EPR techniques and their recent applications for answering structure and conformational dynamics related questions of important membrane protein systems.
Databáze:	Directory of Open Access Journals
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